pTurboGFP-B vector (V011916)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V011916	pTurboGFP-B	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

- - Vector Name:
  - pTurboGFP-B
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 4103 bp
- - Type:
  - Fluorescent Protein Genes & Plasmids
- - Replication origin:
  - ori
- - Source/Author:
  - Evrogen
- - Copy Number:
  - High copy number
- - Promoter:
  - T5

pTurboGFP-B vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTurboGFP-B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTurboGFP-B.        4103 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing TurboGFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pTurboGFP-B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..825
                     /label=TurboGFP
                     /note="improved green fluorescent protein from Pontellina 
                     plumata (Evdokimov et al., 2006)"
     terminator      849..943
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             987..1643
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1711..1797
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1953..2093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2279..2867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3041..3898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3899..4003)
                     /label=AmpR promoter