LOCUS       pTurboGFP-B.        4103 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing TurboGFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pTurboGFP-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..825
                     /label=TurboGFP
                     /note="improved green fluorescent protein from Pontellina 
                     plumata (Evdokimov et al., 2006)"
     terminator      849..943
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             987..1643
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1711..1797
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1953..2093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2279..2867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3041..3898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3899..4003)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aactatgaga
      121 ggatcgggat ccgagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
      181 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccgag
      241 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
      301 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
      361 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
      421 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
      481 atcggcgact tcaaggtgat gggcaccggc ttccccgagg acagcgtgat cttcaccgac
      541 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgatctg
      601 gatggcagct tcacccgcac cttcagcctg cgcgacggcg gctactacag ctccgtggtg
      661 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
      721 atgttcgcct tccgccgcgt ggaggaggat cacagcaaca ccgagctggg catcgtggag
      781 taccagcacg ccttcaagac cccggatgca gatgccggtg aagaatgaag cttaattagc
      841 tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
      901 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagct agcttggcga
      961 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1021 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1081 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1141 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1201 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1261 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     1321 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1381 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     1441 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1501 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1561 atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1621 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc
     1681 ctggggtaat gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac
     1741 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1801 ccgctctaga gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     1861 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     1921 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     1981 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     2041 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     2101 ccgcttcctc gctcactgac tcgctgcgct cggtctgtcg gctgcggcga gcggtatcag
     2161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2461 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3061 cacctatctc agcgatctgt ctatttcgtt catccatagc tgcctgactc cccgtcgtgt
     3121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4081 tcacgaggcc ctttcgtctt cac
//
