Cart 2

pTurboYFP-B vector (V011908)

Basic Vector Information

Vector Name:
pTurboYFP-B
Antibiotic Resistance:
Ampicillin
Length:
4140 bp
Type:
Fluorescent Protein Genes & Plasmids
Replication origin:
ori
Source/Author:
Evrogen
Copy Number:
High copy number
Promoter:
T5

pTurboYFP-B vector Vector Map

pTurboYFP-B4140 bp60012001800240030003600T5 promoterlac operatorATGTurboYFPlambda t0 terminatorCmRrrnB T1 terminatorbomoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTurboYFP-B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTurboYFP-B.        4140 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing TurboYFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pTurboYFP-B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..861
                     /label=TurboYFP
                     /note="enhanced derivative of PhiYFP"
     terminator      886..980
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1024..1680
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1748..1834
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1990..2130
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2316..2904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3078..3935)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3936..4040)
                     /label=AmpR promoter

This page is informational only.