LOCUS       pTurboYFP-B.        4140 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing TurboYFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pTurboYFP-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..861
                     /label=TurboYFP
                     /note="enhanced derivative of PhiYFP"
     terminator      886..980
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1024..1680
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1748..1834
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1990..2130
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2316..2904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3078..3935)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3936..4040)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aactatgaga
      121 ggatcgggat ccatgagcag cggcgccctg ctgttccacg gcaagatccc ctacgtggtg
      181 gagatggagg gcaatgtgga tggccacacc ttcagcatcc gcggcaaggg ctacggcgat
      241 gccagcgtgg gcaaggtgga tgcccagttc atctgcacca ccggcgatgt gcccgtgccc
      301 tggagcaccc tggtgaccac cctgacctac ggcgcccagt gcttcgccaa gtacggcccc
      361 gagctgaagg atttctacaa gagctgcatg cccgatggct acgtgcagga gcgcaccatc
      421 accttcgagg gcgatggcaa tttcaagacc cgcgccgagg tgaccttcga gaatggcagc
      481 gtgtacaatc gcgtgaagct gaatggccag ggcttcaaga aggatggcca cgtgctgggc
      541 aagaatctgg agttcaattt caccccccac tgcctgtaca tctggggcga tcaggccaat
      601 cacggcctga agagcgcctt caaaatctgc cacgagatca ccggcagcaa gggcgatttc
      661 atcgtggccg atcacaccca gatgaatacc cccatcggcg gcggccccgt gcacgtgccc
      721 gagtaccacc acatgagcta ccacgtgaag ctgagcaagg atgtgaccga tcaccgcgat
      781 aatatgagcc tgaaggagac cgtgcgcgcc gtggattgcc gcaagaccta cgacttcgac
      841 gccggttccg gagatacaag ctgaaagctt aattagctga gcttggactc ctgttgatag
      901 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
      961 cgttttttat tggtgagaat ccaagctagc ttggcgagat tttcaggagc taaggaagct
     1021 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1081 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1141 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1201 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
     1261 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1321 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1381 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1441 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1501 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1561 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
     1621 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     1681 taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt
     1741 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1801 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ctctagagct gcctcgcgcg
     1861 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1921 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     1981 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     2041 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     2101 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     2161 ctgcgctcgg tctgtcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2221 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2281 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2341 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2401 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2461 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     2521 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2581 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2641 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2701 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2761 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2821 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2941 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3001 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3061 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3121 tttcgttcat ccatagctgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3181 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4081 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
//
