Lafmid BA vector (V011764)

MSDS

Basic Vector Information

- - Vector Name:
  - Lafmid BA
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3152 bp
- - Type:
  - I.M.A.G.E. Consortium Plasmids
- - Replication origin:
  - ori
- - Source/Author:
  - I.M.A.G.E. Consortium
- - Copy Number:
  - High copy number
- - 5' Primer:
  - M13 fwd
- - 3' Primer:
  - M13 rev

Lafmid BA vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Lafmid BA vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Lafmid_BA.        3152 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Phagemid vector used for cDNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    Lafmid BA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..555)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1042..1058
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1070..1101
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(1114..1130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1138..1154)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1162..1192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1207..1228)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1516..2104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2278..3135)
                     /label=AmpR
                     /note="beta-lactamase"

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