LOCUS       Lafmid_BA.        3152 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Phagemid vector used for cDNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    Lafmid BA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..555)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1042..1058
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1070..1101
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(1114..1130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1138..1154)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1162..1192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1207..1228)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1516..2104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2278..3135)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 cgatgaacgg taatcgtaaa actagcatgt caatcatatg taccccggtt gataatcaga
       61 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaaac gttaatattt
      121 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
      181 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
      241 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      301 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
      361 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      421 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
      481 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
      541 cgctacaggg cgcgtactat ggttgctttg acgagcacgt ataacgtgct ttcctcgtta
      601 gaatcagagc gggagctaaa caggaggccg attaaaggga ttttagacag gaacggtacg
      661 ccagaatcct gagaagtgtt tttataatca gtgaggccac cgagtaaaag agtctgtcca
      721 tcacgcaaat taaccgttgt cgcaatactt ctttgattag taataacatc acttgcctga
      781 gtagaagaac tcaaactatc ggccttgctg gtaatatcca gaacaatatt accgccagcc
      841 attgcaacag gaaaaacgct catggaaata cctacatttt gacgctcaat cgtctggaat
      901 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      961 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1021 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attccccttg cggccgcaag
     1081 gggatccgtc gagccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1141 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1201 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1261 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1321 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1381 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1441 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1501 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1561 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1621 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1681 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     1741 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1801 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1861 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1921 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     1981 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2041 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2101 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2161 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2221 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2281 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2341 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2401 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2461 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2521 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2581 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     2641 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     2701 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2761 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2821 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2881 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2941 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3001 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3061 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3121 atgttgaata ctcatactct tcctttttca at
//
