pExpress-1 vector (V011719)

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Basic Vector Information

Vector Name:: pExpress-1

Antibiotic Resistance:: Ampicillin

Length:: 4117 bp

Type:: I.M.A.G.E. Consortium Plasmids

Replication origin:: ori

Source/Author:: I.M.A.G.E. Consortium

Copy Number:: High copy number

Promoter:: CMV

5' Primer:: M13 fwd

3' Primer:: M13 rev

pExpress-1 vector Map

Copy Sequence View Map

pExpress-1 vector Sequence

LOCUS       40924_18936        4117 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pExpress-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Gruber CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) Express Genomics, Inc., 5 South Wisner 
            Street, Frederick, MD 21701, USA
REFERENCE   2  (bases 1 to 4117)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4117)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2006) Express Genomics, Inc., 5 South Wisner Street, 
            Frederick, MD 21701, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(125..259)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     664..680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(772..790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(801..817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(943..1146)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1147..1450)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1910..2498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2672..3529)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3530..3634)
                     /label=AmpR promoter
     rep_origin      complement(3661..4116)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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