LOCUS       40924_18936        4117 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pExpress-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Gruber CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) Express Genomics, Inc., 5 South Wisner 
            Street, Frederick, MD 21701, USA
REFERENCE   2  (bases 1 to 4117)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4117)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2006) Express Genomics, Inc., 5 South Wisner Street, 
            Frederick, MD 21701, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(125..259)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     664..680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(772..790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(801..817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(943..1146)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1147..1450)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1910..2498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2672..3529)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3530..3634)
                     /label=AmpR promoter
     rep_origin      complement(3661..4116)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac
      661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactcctc
      721 gaggttaatt aagcggccgc gatatcccgg gaattccgga ccgaaacttg ttctatagtg
      781 tcacctaaat aggcctaatg gtcatagctg tttcctgtgt gaaattgtta tccgctccgc
      841 ggcctaggct agagtccgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
      901 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac
      961 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     1021 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     1081 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     1141 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     1201 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     1261 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     1321 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     1381 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     1441 ttatgtaacg acatgcatct aatgagtgaa agggcctcgt actacgccta tttttatagg
     1501 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1561 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1621 aataaccctg ataaatgctt caataatatt gaaaaacgcg cgaattgcaa gctctgcatt
     1681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2101 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2341 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3661 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3721 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3781 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3841 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3901 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3961 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4021 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4081 cacccgccgc gcttaatgcg ccgctacagg gcgcgtc
//
