Basic Vector Information
- Vector Name:
- pOTB7-3
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 2816 bp
- Type:
- I.M.A.G.E. Consortium Plasmids
- Replication origin:
- ori
- Source/Author:
- I.M.A.G.E. Consortium
- Copy Number:
- High copy number
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pOTB7-3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOTB7-3 vector Sequence
LOCUS pOTB7-3. 2816 bp DNA circular SYN 01-JAN-1980
DEFINITION cDNA cloning vector with an f1 origin derived from pOTB7.
ACCESSION .
VERSION .
KEYWORDS pOTB7-3.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2816)
AUTHORS I.M.A.G.E. Consortium
TITLE Direct Submission
REFERENCE 2 (bases 1 to 2816)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT The presence in pOTB7-3 of a toxic ccdB gene flanked by BstXI sites
is described here:
http://www.imageconsortium.org/?i=vectors#171
However, this gene was not detected in the sequence provided.
FEATURES Location/Qualifiers
source 1..2816
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 53..71
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
protein_bind 85..109
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
primer_bind 111..127
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 444..465
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 480..510
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 518..534
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 542..558
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 576..594
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(746..762)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(763..787)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(819..837)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(890..1345)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 1416..2004
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 2145..2801
/label=CmR
/note="chloramphenicol acetyltransferase"
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