LOCUS       pOTB7-3.        2816 bp DNA     circular SYN 01-JAN-1980
DEFINITION  cDNA cloning vector with an f1 origin derived from pOTB7.
ACCESSION   .
VERSION     .
KEYWORDS    pOTB7-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The presence in pOTB7-3 of a toxic ccdB gene flanked by BstXI sites 
            is described here:
            http://www.imageconsortium.org/?i=vectors#171
            However, this gene was not detected in the sequence provided.
FEATURES             Location/Qualifiers
     source          1..2816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    85..109
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    444..465
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        480..510
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    518..534
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     542..558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        576..594
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(746..762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(763..787)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(819..837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(890..1345)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1416..2004
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2145..2801
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
       61 gacactatag aactcgaagg atccacaagt ttgtacaaaa aagcaggctt gtaaaacgac
      121 ggccagtaac tataacggtc ctaaggtagc gaggcctggg tggcgaattc ccttactagt
      181 ttccctcgag cggccgccag tgtgctggaa agcatgttct ttcctgcgtt atcccctgat
      241 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      301 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      361 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      421 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      481 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      541 acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta tagaatactc
      601 aagctatgca tcaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      661 ggaattctgc agatatccat cacactggcg gccgctcgag gcatctatgt cgggtgcgga
      721 gaaagaggta atgaaatggc acatggtcat agctgtttcc tgacccagct ttcttgtaca
      781 aagtggtaga tctaaggcgc gcctgatctg ccggtctccc tatagtgagt cgtattaatt
      841 tcgataagcc aggttaacct gcattaatga atcggctgca gtacccggga aattgtaagc
      901 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      961 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1021 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1081 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     1141 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     1201 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     1261 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1321 cttaatgcgc cgctacaggg cgcgtcccat tcgccattcc cgggaattta acccgcctaa
     1381 tgagcgggct tttttttgtg atccaaagga tcttcttgag atcctttttt tctgcgcgta
     1441 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1501 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1561 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1621 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1681 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1741 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1801 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1861 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1921 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1981 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg atcacaacaa aaagcccgct
     2041 cattaggcgg gctaaattct catgtttgac agcttatcat cgataagcta gcggccgcta
     2101 gctttaatga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     2161 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     2221 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     2281 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2341 tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     2401 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     2461 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2521 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2581 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2641 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2701 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     2761 atgaattaca acagtactgc gatgagtggc agggcggggc gtaattggta cgtcga
//
