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pOTB7 vector (V011716)

Basic Vector Information

Vector Name:
pOTB7
Antibiotic Resistance:
Chloramphenicol
Length:
1815 bp
Type:
I.M.A.G.E. Consortium Plasmids
Replication origin:
ori
Source/Author:
Berkeley Drosophila Genome Project
Copy Number:
High copy number
5' Primer:
M13 fwd
3' Primer:
M13 rev

pOTB7 vector Map

Copy Sequence View Map
pOTB71815 bp60012001800SP6 promoterattB1M13 fwdM13 revattB2T7 promoteroriCmR

pOTB7 vector Sequence

LOCUS       40924_33952        1815 bp DNA     circular SYN 01-JAN-1980
DEFINITION  cDNA cloning vector with a Gateway(R) recombination cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Berkeley Drosophila Genome Project
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..1815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    85..109
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(236..252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(253..277)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(293..311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      415..1003
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1144..1800
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"

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