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pOTB7 vector (V011716)

Basic Vector Information

Vector Name:
pOTB7
Antibiotic Resistance:
Chloramphenicol
Length:
1815 bp
Type:
I.M.A.G.E. Consortium Plasmids
Replication origin:
ori
Source/Author:
Berkeley Drosophila Genome Project
Copy Number:
High copy number
5' Primer:
M13 fwd
3' Primer:
M13 rev

pOTB7 vector Vector Map

pOTB71815 bp60012001800SP6 promoterattB1M13 fwdM13 revattB2T7 promoteroriCmR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pOTB7 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33952        1815 bp DNA     circular SYN 01-JAN-1980
DEFINITION  cDNA cloning vector with a Gateway(R) recombination cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Berkeley Drosophila Genome Project
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..1815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    85..109
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(236..252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(253..277)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(293..311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      415..1003
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1144..1800
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"

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