LOCUS       40924_33952        1815 bp DNA     circular SYN 01-JAN-1980
DEFINITION  cDNA cloning vector with a Gateway(R) recombination cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Berkeley Drosophila Genome Project
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..1815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    85..109
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(236..252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(253..277)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(293..311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      415..1003
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1144..1800
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
       61 gacactatag aactcgaagg atccacaagt ttgtacaaaa aagcaggctt gtaaaacgac
      121 ggccagtaac tataacggtc ctaaggtagc gaggcctggg tggcgaattc ccttactagt
      181 ttccctcgag gcatctatgt cgggtgcgga gaaagaggta atgaaatggc acatggtcat
      241 agctgtttcc tgacccagct ttcttgtaca aagtggtaga tctgccggtc tccctatagt
      301 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc tgcagtaccc
      361 gggaatttaa cccgcctaat gagcgggctt ttttttgtga tccaaaggat cttcttgaga
      421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgga
     1021 tcacaacaaa aagcccgctc attaggcggg ctaaattctc atgtttgaca gcttatcatc
     1081 gataagctag cggccgctag ctttaatgag ttatcgagat tttcaggagc taaggaagct
     1141 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1201 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1261 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1321 attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc aatgaaagac
     1381 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1441 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1501 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1561 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1621 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1681 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     1741 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     1801 taattggtac gtcga
//
