pT7T3D-PacI vector (V011710)

LOCUS       pT7T3D-PacI.        2897 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Directional cDNA cloning vector. Also known as pT7T3-Pac, pT7T3Pac, 
            pT7T3D-Pac, or pT7T3D.
ACCESSION   .
VERSION     .
KEYWORDS    pT7T3D-PacI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   I.M.A.G.E. Cpnsortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        169..187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    192..259
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(266..284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(299..315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(323..339)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(347..377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(392..413)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(701..1289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1463..2320)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2321..2425)
                     /label=AmpR promoter