LOCUS       pT7T3D-PacI.        2897 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Directional cDNA cloning vector. Also known as pT7T3-Pac, pT7T3Pac, 
            pT7T3D-Pac, or pT7T3D.
ACCESSION   .
VERSION     .
KEYWORDS    pT7T3D-PacI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   I.M.A.G.E. Cpnsortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        169..187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    192..259
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(266..284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(299..315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(323..339)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(347..377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(392..413)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(701..1289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1463..2320)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2321..2425)
                     /label=AmpR promoter
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctaaaa ttaaccctca
      181 ctaaagggaa taagcttgcg gccgcttaat taagacggat ccccgactac gtagtcggga
      241 attcttggcc tcgagggcca aattccctat agtgagtcgt attaaattcg taatcatggt
      301 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      361 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      421 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      481 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      541 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      601 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      661 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      721 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      781 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      841 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
      901 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      961 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1021 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1081 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1141 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1201 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1261 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1321 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1381 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1441 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1501 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1561 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1621 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1681 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1741 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1801 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1861 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1921 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1981 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2041 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2101 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2161 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2221 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2281 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa
     2461 acgttaatgt tttgttaaat ttcgcgttaa atatttgtta aatcagctta ttttttaacc
     2521 agtaagcaga aaatgacaaa aatccttata aatcaaaaga atagaccgag ttagttgtga
     2581 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtaaaac
     2641 cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt tttggaggtc
     2701 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2761 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2821 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2881 gccgctactg ggcgcgt
//
