pBAC-2cp vector (V011672)

LOCUS       pBAC-2cp.        5411 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the polyhedrin promoter, encoding 
            an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC-2cp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1168..1259
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1266..1268
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1272..1289
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1299..1316
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1320..1364
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1380..1394
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1397..1460
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1461..1478
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     1515..2872
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3135..3723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3897..4754)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4755..4859)
                     /label=AmpR promoter
     rep_origin      4886..5341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"