LOCUS       pBAC-2cp.        5411 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the polyhedrin promoter, encoding 
            an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC-2cp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1168..1259
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1266..1268
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1272..1289
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1299..1316
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1320..1364
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1380..1394
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1397..1460
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1461..1478
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     1515..2872
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3135..3723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3897..4754)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4755..4859)
                     /label=AmpR promoter
     rep_origin      4886..5341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aaccatctcg
     1201 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     1261 tgcccatggt ccatcatcac caccatcact ccgcgggttt ggtgcccaga ggcagcggca
     1321 aagaaacggc ggcggcgaaa tttgaacgcc aacacatgga cagcgctagc ggtggaggtg
     1381 atgacgacga caagagcccg ggcttctcct caaaaggcct ggatccgaat tcgagctcga
     1441 agcttgcggc cgcactcgag caccaccacc accaccacta acctaggtag ctgagcgcat
     1501 gcaagctgat ccgggttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     1561 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     1621 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     1681 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     1741 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     1801 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     1861 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     1921 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt
     1981 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
     2041 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
     2101 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
     2161 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
     2221 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
     2281 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
     2341 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
     2401 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
     2461 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
     2521 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
     2581 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
     2641 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
     2701 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
     2761 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
     2821 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg gatcgatccc
     2881 gcacgccgat aacaagcctt ttcattttta ctacagcatt gtagtggcga gacacttcgc
     2941 tgtcgtcgag gtttaaacgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3001 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3061 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3121 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3181 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3241 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3301 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3361 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3421 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3481 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3541 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3601 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3841 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3901 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3961 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4021 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4081 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4141 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4201 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4261 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4321 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4381 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4441 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4501 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4561 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4621 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4681 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4741 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4861 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5341 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     5401 ctattacgcc a
//
