pBACgus-5 vector (V011664)

LOCUS       pBACgus-5.        7666 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the gp64 promoter, encoding 
            encoding beta-glucuronidase plus an N-terminal 
            6xHis-thrombin-S-Tag-enterokinase cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3315..3518
                     /label=gp64 promoter
                     /note="promoter for the baculovirus gp64 glycoprotein gene"
     CDS             3521..3523
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             3527..3544
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             3554..3571
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             3575..3619
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             3635..3649
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3651..3715
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3716..3733
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     3770..5127
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5390..5978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6152..7009)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7010..7114)
                     /label=AmpR promoter
     rep_origin      7141..7596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"