LOCUS       pBACgus-5.        7666 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the gp64 promoter, encoding 
            encoding beta-glucuronidase plus an N-terminal 
            6xHis-thrombin-S-Tag-enterokinase cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3315..3518
                     /label=gp64 promoter
                     /note="promoter for the baculovirus gp64 glycoprotein gene"
     CDS             3521..3523
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             3527..3544
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             3554..3571
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             3575..3619
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             3635..3649
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3651..3715
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3716..3733
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     3770..5127
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5390..5978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6152..7009)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7010..7114)
                     /label=AmpR promoter
     rep_origin      7141..7596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgtcga ctgagcgtcc gtgttcatga tcccgttttt ataacagcca
     3361 gataaaaata atcttatcaa ttaagataaa aagataagat tattaatcta acaacgtgcc
     3421 ttgtgtcacg taggccagat aacggtcggg tatataagac gcctcaacgc tactagtaaa
     3481 tcagtcacac caaggcttca ataaggaaca cacaagcacc atggtccatc atcaccacca
     3541 tcactccgcg ggtttggtgc ccagaggcag cggcaaagaa acggcggcgg cgaaatttga
     3601 acgccaacac atggacagcg ctagcggtgg aggtgatgac gacgacaaga gcccgggctt
     3661 ctcctcaaaa ggcctggatc cgaattcgag ctcgaagctt gcggccgcac tcgagcacca
     3721 ccaccaccac cactaaccta ggtagctgag cgcatgcaag ctgatccggg ttattagtac
     3781 atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg tacgtatttt
     3841 aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat caaatgattt
     3901 tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta aaataggttt
     3961 cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc taatggattt
     4021 tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt gtcgatattc
     4081 gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg cgcttttgta
     4141 tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt aaatagagct
     4201 tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa
     4261 ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg acgcctatta
     4321 attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg aattatttct
     4381 gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa ttcagacaac
     4441 acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc tactaatggc
     4501 ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg ggctggcggc
     4561 ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc aaatgtctct
     4621 ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt ttttggtttg
     4681 accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa ttgttgtctg
     4741 tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg cggcaattca
     4801 gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg agaaggtggt
     4861 ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac gattgtgggc
     4921 accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg cagcgcttgg
     4981 ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc tataagcatt
     5041 gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta agcaattgta
     5101 ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa gccttttcat
     5161 ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgaggttta aacgcttcct
     5221 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5281 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5581 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5821 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6181 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6241 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6301 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6361 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6421 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6481 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6541 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6601 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6661 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6721 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6781 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6841 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6901 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6961 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7021 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7081 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7561 acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca
     7621 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgcca
//
