pBACsurf-1 vector (V011659)

LOCUS       V011659                 9429 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011659
VERSION     V011659
KEYWORDS    pBACsurf-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     2254..2313
                     /label="GP64 signal sequence"
                     /note="signal sequence from the baculovirus envelope
                     glycoprotein GP64"
     misc_feature    2317..2332
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2332..3807
                     /label="GP64 mature domain"
                     /note="mature domain of the baculovirus envelope
                     glycoprotein GP64"
     misc_recomb     3819..5176
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     CDS             5469..6284
                     /gene="PK1"
                     /label="Serine/threonine-protein kinase 1"
                     /note="Serine/threonine-protein kinase 1 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41415"
     primer_bind     complement(6324..6340)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6553..7008
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7290..7394
                     /label="AmpR promoter"
     CDS             7395..8252
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8426..9014
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9302..9323
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9338..9368
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9376..9392
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9400..9416
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"