LOCUS       V011659                 9429 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011659
VERSION     V011659
KEYWORDS    pBACsurf-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     2254..2313
                     /label="GP64 signal sequence"
                     /note="signal sequence from the baculovirus envelope
                     glycoprotein GP64"
     misc_feature    2317..2332
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2332..3807
                     /label="GP64 mature domain"
                     /note="mature domain of the baculovirus envelope
                     glycoprotein GP64"
     misc_recomb     3819..5176
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     CDS             5469..6284
                     /gene="PK1"
                     /label="Serine/threonine-protein kinase 1"
                     /note="Serine/threonine-protein kinase 1 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41415"
     primer_bind     complement(6324..6340)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6553..7008
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7290..7394
                     /label="AmpR promoter"
     CDS             7395..8252
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8426..9014
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9302..9323
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9338..9368
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9376..9392
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9400..9416
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatcta tgctactagt aaatcagtca
     2221 caccaaggct tcaataagga acacacaagc aagatggtaa gcgctattgt tttatatgtg
     2281 cttttggcgg cggcggcgca ttctgccttt gcggatctgc aggtaccccg ggcggagcac
     2341 tgcaacgcgc aaatgaagac gggtccgtac aagattaaaa acttggacat taccccgccc
     2401 aaggaaacgc tgcaaaagga cgtggaaatc accatcgtgg agacggacta caacgaaaac
     2461 gtgattatcg gctacaaggg gtactaccag gcgtatgcgt acaacggcgg ctcgctggat
     2521 cccaacacac gcgtcgaaga aaccatgaaa acgctgaatg tgggcaaaga ggatttgctt
     2581 atgtggagca tcaggcagca gtgcgaggtg ggcgaagagc tgatcgaccg ttggggcagt
     2641 gacagcgacg actgttttcg cgacaacgag ggccgcggcc agtgggtcaa aggcaaagag
     2701 ttggtgaagc ggcagaataa caatcacttt gcgcaccaca cgtgcaacaa atcgtggcga
     2761 tgcggcattt ccacttcgaa aatgtacagc aggctcgagt gccaggacga cacggacgag
     2821 tgccaggtat acattttgga cgctgagggc aaccccatca acgtgaccgt ggacactgtg
     2881 cttcatcgag acggcgtgag tatgattctc aaacaaaagt ctacgttcac cacgcgccaa
     2941 ataaaagctg cgtgtctgct cattaaagat gacaaaaata accccgagtc ggtgacacgc
     3001 gaacactgtt tgattgacaa tgatatatat gatctttcta aaaacacgtg gaactgcaag
     3061 tttaacagat gcattaaacg caaagtcgag caccgagtca agaagcggcc gcccacttgg
     3121 cgccacaacg ttagagccaa gtacacagag ggagacactg ccaccaaagg cgacctgatg
     3181 catattcaag aggagctgat gtacgaaaac gatttgctga aaatgaacat tgagctgatg
     3241 catgcgcaca tcaacaagct aaacaatatg ctgcacgacc tgatagtctc cgtggccaag
     3301 gtggacgagc gtttgattgg caatctcatg aacaactctg tttcttcaac atttttgtcg
     3361 gacgacacgt ttttgctgat gccgtgcacc aatccgccgg cacacaccag taattgctac
     3421 aacaacagca tctacaaaga agggcgttgg gtggccaaca cggactcgtc gcaatgcata
     3481 gattttagca actacaagga actagcaatt gacgacgacg tcgagttttg gatcccgacc
     3541 atcggcaaca cgacctatca cgacagttgg aaagatgcca gcggctggtc gtttattgcc
     3601 caacaaaaaa gcaacctcat aaccaccatg gagaacacca agtttggcgg cgtcggcacc
     3661 agtctgagcg acatcacttc catggctgaa ggcgaattgg ccgctaaatt gacttcgttc
     3721 atgtttggtc atgtagttaa ctttgtaatt atattaattg tgattttatt tttgtactgt
     3781 atgattagaa accgtaatag acaatattaa agatccgggt tattagtaca tttattaagc
     3841 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt
     3901 aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt
     3961 atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc
     4021 aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc
     4081 cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt
     4141 gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc
     4201 actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt
     4261 aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt
     4321 agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc
     4381 taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg
     4441 tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag
     4501 cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg
     4561 agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg
     4621 cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac
     4681 agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag
     4741 acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg
     4801 tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg
     4861 tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc
     4921 cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg
     4981 cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa
     5041 ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct
     5101 atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga
     5161 ttgtctcaag ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag
     5221 cattgtagtg gcgagacact tcgctgtcgt cgacgtacat gtatgctttg ttgtcaaaaa
     5281 cgtcgttggc aagctttaaa atatttaaaa gaacatctct gttcagcacc actgtgttgt
     5341 cgtaaatgtt gtttttgata atttgcgctt ccgcagtatc gacacgttca aaaaattgat
     5401 gcgcatcaat tttgttgttc ctattattga ataaataaga ttgtacagat tcatatctac
     5461 gattcgtcat ggccaccaca aatgctacgc tgcaaacgct ggtacaattt tacgaaaact
     5521 gcaaaaacgt caaaactcgg tataaaataa tcaacgggcg ctttggcaaa atatctattt
     5581 tatcgcacaa gcccactagc aaattgtatt tgcagaaaac aatttcggcg cacaatttta
     5641 acgctgacga aataaaagtt caccagttaa tgagcgacca cccaaatttt ataaaaatct
     5701 attttaatca cggttccatc aacaaccaag tgatcgtgat ggactacatt gactgtcccg
     5761 atttatttga aacactacaa attaaaggcg agctttcgta ccaacttgtt agcaatatta
     5821 ttagacagct gtgtgaagcg ctcaacgatt tgcacaagca caatttcata cacaacgaca
     5881 taaaactcga aaatgtctta tatttcgaag cacttgatcg cgtgtatgtt tgcgattacg
     5941 gattgtgcaa acacgaaaac tcacttagcg tgcacgacgg cacgttggag tattttagtc
     6001 cggaaaaaat tcgacaccac aactatgcac gttcgtttga ctggtacgcc gtcggcgtgt
     6061 taacatacaa gttgctaacc ggcggccgac acccatttga aaaaagcgaa gacgaaatgt
     6121 tggacttgaa tagcatgaag cgtcgtcagc aatacaatga cattggcgtt ttaaaacacg
     6181 ttcgtaacgt taacgctcgt gactttgtgt actgcctaac aagatacaac atagattgta
     6241 gactcacaaa ttacaaacaa attataaaac atgagttttt gtcgtaaaaa tgccacttgt
     6301 tttacgagta gaattagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6361 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6421 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6481 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     6541 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     6781 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6841 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     6901 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6961 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     7021 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7081 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7141 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7201 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7261 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7921 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     8581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
     9421 ttacgaatt
//
