LOCUS       Exported                5237 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  83..248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    277..411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(670..761)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        785..894
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     polyA_signal    1030..1078
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     promoter        1586..1614
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1803..2336
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2403..2627
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(2932..3520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3691..4551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4552..4656)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4682..5136)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aaagtagccg aagatgacgg tttgtcacat ggagttggca ggatgtttga ttaaaaacat
       61 aacaggaaga aaaatgcccc gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg
      121 aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta
      181 gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt
      241 atctggttcg gatctcctag gctcaagcag tgatcagatc cagacatgat aagatacatt
      301 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      361 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
      421 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
      481 taaaacctct acaaatgtgg tatggctgat tatgatcctc tagtacttct cgacaagctt
      541 gtcgagactg caggctctag attcgaaagc ggccgcgact agtgagctcg tcgacgtagg
      601 cctttgaatt ccgcgcgctt cggaccggga tccgcgcccg atggtgggac ggtatgaata
      661 atccggaata tttataggtt tttttattac aaaactgtta cgaaaacagt aaaatactta
      721 tttatttgcg agatggttat cattttaatt atctccatga tctattaata ttccggagta
      781 tacggacctt taattcaacc caacacaata tattatagtt aaataagaat tattatcaaa
      841 tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac aatcactcga
      901 cgaagacttg atcacccggg atctcgagcc atggtgctag cagctgatgc atagcatgcg
      961 gtaccgggag atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     1021 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     1081 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     1141 gaccaatacg cccgcgtttc ttccttttcc ccaccccaac ccccaagttc gggtgaaggc
     1201 ccagggctcg cagccaacgt cggggcggca agccctgcca tagccactac gggtacgtag
     1261 ccaaccacta gaactatagc tagagtcctg ggcgaacaaa cgatgctcgc cttccagaaa
     1321 accgaggatg cgaaccactt catccggggt cagcaccacc ggcaagcgcc gcgacggccg
     1381 aggtctaccg atctcctgaa gcaagggcag atccgtgcac agcaccttgc cgtagaagaa
     1441 cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag
     1501 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
     1561 gaaacggatg aaggcacgaa cccagttgac ataagcctgt tcggttcgta aactgtaatg
     1621 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1681 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg
     1741 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1801 cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
     1861 gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca
     1921 tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac
     1981 atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg
     2041 ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg
     2101 agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc
     2161 agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt
     2221 atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt
     2281 tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat
     2341 tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg tcacaacgcc
     2401 gcgaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac gggcaatttg
     2461 cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca
     2521 gcataactgg actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat
     2581 agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag
     2641 ggcatccatt tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtctatgcg
     2701 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     2761 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2821 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4681 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4741 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4801 atagggttga gtgttgttcc agtttggaac aagagtccac tattaagaac gtggactcca
     4861 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4921 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4981 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5041 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5101 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcaaa
     5161 taagcgttga tattcagtca attacaaaca ttaataacga agagatgaca gaaaaatttt
     5221 cattctgtga cagagaa
//
