LOCUS       Exported                4776 bp DNA     circular SYN 03-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        178..269
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    540..674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  703..868
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1052..1506
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1532..1636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1637..2497
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2668..3256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  3561..3785
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3852..4385)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4574..4602)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct
       61 gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc
      121 ccaggactct agctatagtt ctagtggttg gctacgtata ctccggaata ttaatagatc
      181 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
      241 acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
      301 ggcgcggatc ccggtccgaa gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag
      361 tcgcggccgc tttcgaatct agagcctgca gtctcgaggc atgcggtacc aagcttgtcg
      421 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      481 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      661 atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc
      721 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
      781 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
      841 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa
      901 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca
      961 cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca
     1021 acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     1081 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1141 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1201 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1261 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1321 ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga acaacactca
     1381 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     1441 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     1501 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1561 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1621 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1681 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1741 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1801 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1861 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1921 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1981 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2041 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2101 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2161 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2221 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2281 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2341 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2401 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2461 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2521 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2581 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2701 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3421 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3481 ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     3541 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     3601 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     3661 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     3721 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta
     3781 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
     3841 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     3901 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     3961 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     4021 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     4081 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     4141 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     4201 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     4261 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     4321 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     4381 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     4441 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     4501 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     4561 ctacttgcat tacagttttc gaaccgaaca ggcttatgtc aactgggttc gtgccttcat
     4621 ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct
     4681 gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc
     4741 cttgctgttc ttctacggca aggtgctgtg cacgga
//
