LOCUS       Exported                4856 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        224..315
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             382..399
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             421..441
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV Site
                     /translation="ENLYFQG"
     polyA_signal    666..800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  829..994
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1178..1632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1658..1762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1763..2623
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2794..3382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  3687..3911
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3978..4511)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4700..4728)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
       61 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
      121 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
      181 atagttctag tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa
      241 aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata
      301 aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg cggatctcgg
      361 tccgaaacca tgtcgtacta ccatcaccat caccatcacg attacgatat cccaacgacc
      421 gaaaacctgt attttcaggg cgccatggat ccggaattca aaggcctacg tcgacgagct
      481 caactagtgc ggccgctttc gaatctagag cctgcagtct cgaggcatgc ggtaccaagc
      541 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
      601 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      661 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      781 tatcttatca tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt
      841 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
      901 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
      961 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     1021 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     1081 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     1141 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1201 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1261 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1321 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1381 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1441 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     1501 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     1621 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1981 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3481 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3541 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     3601 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     3661 ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     3721 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     3781 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     3841 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
     3901 agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc
     3961 tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct
     4021 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc
     4081 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg
     4141 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca
     4201 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct
     4261 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg
     4321 gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca
     4381 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc
     4441 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     4501 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
     4561 ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac
     4621 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     4681 catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc
     4741 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc
     4801 atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcagg
//
