pIEx/Bac-5 vector (V011632)

LOCUS       pIEx_Bac-5.        7499 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell and baculovirus vector encoding a cleavable N-terminal 
            GST tag and a cleavable C-terminal Strep-Tag(R) II.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx Bac-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     enhancer        1196..1678
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        1682..2273
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        2283..2392
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2435..3088
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             3119..3142
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    3140..3184
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3188..3205
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             3212..3235
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      3268..3574
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     misc_recomb     3603..4960
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5223..5811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5985..6842)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6843..6947)
                     /label=AmpR promoter
     rep_origin      6974..7429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"