LOCUS       pIEx_Bac-5.        7499 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell and baculovirus vector encoding a cleavable N-terminal 
            GST tag and a cleavable C-terminal Strep-Tag(R) II.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx Bac-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     enhancer        1196..1678
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        1682..2273
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        2283..2392
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2435..3088
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             3119..3142
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    3140..3184
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3188..3205
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             3212..3235
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      3268..3574
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     misc_recomb     3603..4960
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5223..5811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5985..6842)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6843..6947)
                     /label=AmpR promoter
     rep_origin      6974..7429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
     1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
     1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
     1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
     1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
     1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
     1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
     1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
     1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
     1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
     1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
     1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
     1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
     1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
     1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
     2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
     2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
     2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
     2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
     2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaatccgga
     2401 ttattcatac cgtcccacca tcgggcgcgg atccatggcc cctatactag gttattggaa
     2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     3061 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtcata atcaagcact
     3121 tgaagtcctc tttcagggac ccgggtacca ggatcctgta caagtcgacg cggccgcaga
     3181 gctcgctctg gtgccacgcg gtagttccgc ttggagccac ccgcagttcg aaaagtaagt
     3241 gattaacctc acgacctagg cctcaggtta tacatatatt ttgaatttaa ttaattatac
     3301 atatatttta tattattttt gtcttttatt atcgaggggc cgttgttggt gtggggtttt
     3361 gcatagaaat aacaatggga gttggcgacg ttgctgcgcc aacaccacct cccttccctc
     3421 ctttcatcat gtatctgtag ataaaataaa atattaaacc taaaaacaag accgcgccta
     3481 tcaacaaaat gataggcatt aacttgccgc tgacgctgtc actaacgttg gacgatttgc
     3541 cgactaaacc ttcatcgccc agtaaccaat ctaggtagct gagcgcatgc aagctgatcc
     3601 gggttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg
     3661 ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa
     3721 aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt
     3781 gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact
     3841 atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc
     3901 tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt
     3961 atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac
     4021 gttaaataga gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc
     4081 gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac
     4141 acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt
     4201 tggaattatt tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt
     4261 taattcagac aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa
     4321 atctactaat ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg
     4381 cggggctggc ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg
     4441 ctcaaatgtc tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc
     4501 cgtttttggt ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa
     4561 caattgttgt ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc
     4621 gggcggcaat tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac
     4681 gggagaaggt ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg
     4741 cacgattgtg ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg
     4801 aggcagcgct tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc
     4861 tgctataagc attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat
     4921 gtaagcaatt gtattgtaaa gagattgtct caagctcgga tcgatcccgc acgccgataa
     4981 caagcctttt catttttact acagcattgt agtggcgaga cacttcgctg tcgtcgaggt
     5041 ttaaacgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5641 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     5701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5881 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5941 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6001 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6061 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6121 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6181 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6241 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6301 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6361 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6421 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6481 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6541 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6601 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6661 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6721 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6781 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6841 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6901 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6961 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7321 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7381 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca
     7441 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgcca
//
