pIEx-10 vector (V011628)

LOCUS       pIEx-10.        3801 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in insect cells of secreted 
            proteins with a cleavable N-terminal Strep-Tag(R) II plus a 
            C-terminal 10xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     sig_peptide     1084..1143
                     /label=IgM signal sequence
                     /note="signal sequence from mouse IgM heavy chain"
     CDS             1156..1179
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1186..1200
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1200..1259
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1270..1296
                     /label=9xHis
                     /note="9xHis affinity tag"
     terminator      1318..1625
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1655..1759
                     /label=AmpR promoter
     CDS             1760..2617
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2791..3379
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3667..3688
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3703..3733
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3741..3757
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3765..3781
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"