LOCUS       pIEx-10.        3801 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression in insect cells of secreted 
            proteins with a cleavable N-terminal Strep-Tag(R) II plus a 
            C-terminal 10xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIEx-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     sig_peptide     1084..1143
                     /label=IgM signal sequence
                     /note="signal sequence from mouse IgM heavy chain"
     CDS             1156..1179
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1186..1200
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1200..1259
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1270..1296
                     /label=9xHis
                     /note="9xHis affinity tag"
     terminator      1318..1625
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        1655..1759
                     /label=AmpR promoter
     CDS             1760..2617
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2791..3379
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3667..3688
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3703..3733
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3741..3757
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3765..3781
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatgaaat tcagctgggt catgttcttc ctgatggcag tggttacagg ggtcaattca
     1141 gaggttcagg caagctggag ccacccgcag ttcgaaaagg gtgcagatga cgacgacaag
     1201 gtaccggatc cgacgtcggt cgacaagctt gcggccgcag gagctccggg cttctcctca
     1261 atttccgctc atcaccacca tcatcaccat caccaccact aagtgattaa cctcaggtta
     1321 tacatatatt ttgaatttaa ttaattatac atatatttta tattattttt gtcttttatt
     1381 atcgaggggc cgttgttggt gtggggtttt gcatagaaat aacaatggga gttggcgacg
     1441 ttgctgcgcc aacaccacct cccttccctc ctttcatcat gtatctgtag ataaaataaa
     1501 atattaaacc taaaaacaag accgcgccta tcaacaaaat gataggcatt aacttgccgc
     1561 tgacgctgtc actaacgttg gacgatttgc cgactaaacc ttcatcgccc agtaaccaat
     1621 ctagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1981 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     2281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3421 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3481 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3541 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3601 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3661 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3721 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     3781 ccatgattac gaattcccgg g
//
