pMelBac B vector (V011611)

LOCUS       pMelBac_B.        4821 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expression and secretion of 
            recombinant proteins. For other reading frames, use pMelBac A or 
            pMelBac C.
ACCESSION   .
VERSION     .
KEYWORDS    pMelBac B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Exclusively for use with Bac-N-Blue(TM) DNA.
FEATURES             Location/Qualifiers
     source          1..4821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     96..158
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    158..213
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     225..930
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1506..1610
                     /label=AmpR promoter
     CDS             1611..2468
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2642..3230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3407..4509)
                     /codon_start=3
                     /gene="lacZ (truncated)"
                     /product="N-terminal fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="5' lacZ fragment"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYTDRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQD"
     CDS             complement(4517..4606)
                     /codon_start=1
                     /gene="Autographa californica Ac-pcna"
                     /product="proliferating cell nuclear antigen (PCNA) from 
                     baculovirus"
                     /label=Autographa californica Ac-pcna
                     /note="Ac PCNA"
                     /translation="MHARLNKSSHRRRKISCIKEQLQLESLLKM"
     promoter        complement(4607..4821)
                     /note="ETL promoter"
                     /note="baculovirus early-to-late promoter (Liu et al.,
                     2006)"