LOCUS       pMelBac_B.        4821 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expression and secretion of 
            recombinant proteins. For other reading frames, use pMelBac A or 
            pMelBac C.
ACCESSION   .
VERSION     .
KEYWORDS    pMelBac B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Exclusively for use with Bac-N-Blue(TM) DNA.
FEATURES             Location/Qualifiers
     source          1..4821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     96..158
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    158..213
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     225..930
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1506..1610
                     /label=AmpR promoter
     CDS             1611..2468
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2642..3230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3407..4509)
                     /codon_start=3
                     /gene="lacZ (truncated)"
                     /product="N-terminal fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="5' lacZ fragment"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYTDRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQD"
     CDS             complement(4517..4606)
                     /codon_start=1
                     /gene="Autographa californica Ac-pcna"
                     /product="proliferating cell nuclear antigen (PCNA) from 
                     baculovirus"
                     /label=Autographa californica Ac-pcna
                     /note="Ac PCNA"
                     /translation="MHARLNKSSHRRRKISCIKEQLQLESLLKM"
     promoter        complement(4607..4821)
                     /note="ETL promoter"
                     /note="baculovirus early-to-late promoter (Liu et al.,
                     2006)"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatatgaa attcttagtc aacgttgccc
      121 ttgtttttat ggtcgtatac atttcttaca tctatgcgga tccgagctcg agatctgcag
      181 ctggtaccat ggaattcgaa gcttggagtc gactctgctg aagaggagga aattctcctt
      241 gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct gttcactggt
      301 ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct agattctgtg
      361 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
      421 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
      481 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
      541 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
      601 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
      661 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
      721 tacaattgac tcgacgtaaa cacgttaaat aaagcctgga catatttaac atcgggcgtg
      781 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
      841 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
      901 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
      961 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
     1021 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
     1081 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
     1141 ggtgatgcag acggcggttt aggctcaaat tgtctctttc aggcaacaca gtcggcacct
     1201 caactattgt actggtttcg ggcgtatggt gcactctcag tacaatctgc tctgatgccg
     1261 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     1321 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1381 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1441 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcatcc tgcaccatcg
     3421 tctgctcatc catgacctga ccatgcagag gatgatgctc gtgacggtta acgcctcgaa
     3481 tcagcaacgg cttgccgttc agcagcagca gaccattttc aatccgcacc tcgcggaaac
     3541 cgacatcgca ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg
     3601 cacgatagag attcgggatt tcggcgctcc acagtttcgg gttttcgacg ttcagacgta
     3661 gtgtgacgcg atcggtataa ccaccacgct catcgataat ttcaccgccg aaaggcgcgg
     3721 tgccgctggc gacctgcgtt tcaccctgcc ataaagaaac tgttacccgt aggtagtcac
     3781 gcaactcgcc gcacatctga acttcagcct ccagtacagc gcggctgaaa tcatcattaa
     3841 agcgagtggc aacatggaaa tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt
     3901 cacggaaaat gccgctcatc cgccacatat cctgatcttc cagataactg ccgtcactcc
     3961 aacgcagcac catcaccgcg aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa
     4021 attcagacgg caaacgactg tcctggccgt aaccgaccca gcgcccgttg caccacagat
     4081 gaaacgccga gttaacgcca tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt
     4141 catcaacatt aaatgtgagc gagtaacaac ccgtcggatt ctccgtggga acaaacggcg
     4201 gattgaccgt aatgggatag gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct
     4261 gccagtttga ggggacgacg acagtatcgg cctcaggaag atcgcactcc agccagcttt
     4321 ccggcaccgc ttctggtgcc ggaaaccagg caaagcgcca ttcgccattc aggctgcgca
     4381 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     4441 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     4501 aaacgacggg atctatcatt tttagcagtg attctaattg cagctgctct ttgatacaac
     4561 taattttacg acgacgatgc gagcttttat tcaaccgagc gtgcatgttt gcaatcgtgc
     4621 aagcgttatc aatttttcat tatcgtattg ttgcacatca acaggctgga caccacgttg
     4681 aactcgccgc agttttgcgg caagttggac ccgccgcgca tccaatgcaa actttccgac
     4741 attctgttgc ctacgaacga ttgattcttt gtccattgat cgaagcgagt gccttcgact
     4801 ttttcgtgtc cagtgtggct t
//
