pCLuc-Basic 2 vector (V011559)

LOCUS       pCLuc-Basic_2.        6061 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian promoter reporter vector encoding secreted Cypridina 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pCLuc-Basic 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..68
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             75..1733
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    1745..1793
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      1927..2355
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2369..2699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2766..3557
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3734..3867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3904..3920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3928..3944)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3952..3982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3997..4018)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4306..4894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5068..5925)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5926..6030)
                     /label=AmpR promoter