LOCUS       pCLuc-Basic_2.        6061 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian promoter reporter vector encoding secreted Cypridina 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pCLuc-Basic 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..68
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             75..1733
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    1745..1793
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      1927..2355
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2369..2699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2766..3557
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3734..3867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3904..3920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3928..3944)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3952..3982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3997..4018)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4306..4894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5068..5925)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5926..6030)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcttgg aattctgcag atatcctcga gcccaagctt ggtaccgagc
       61 tcggatccgc caccatgaag accttaattc ttgccgttgc attagtctac tgcgccactg
      121 ttcattgcca ggactgtcct tacgaacctg atccaccaaa cacagttcca acttcctgtg
      181 aagctaaaga aggagaatgt attgatagca gctgtggcac ctgcacgaga gacatactat
      241 cagatggact gtgtgaaaat aaaccaggaa aaacatgttg ccgaatgtgt cagtatgtaa
      301 ttgaatgcag agtagaggcc gcaggatggt ttagaacatt ctatggaaag agattccagt
      361 tccaggaacc tggtacatac gtgttgggtc aaggaaccaa gggcggcgac tggaaggtgt
      421 ccatcaccct ggagaacctg gatggaacca agggggctgt gctgaccaag acaagactgg
      481 aagtggctgg agacatcatt gacatcgctc aagctactga gaatcccatc actgtaaacg
      541 gtggagctga ccctatcatc gccaacccgt acaccatcgg cgaggtcacc atcgctgttg
      601 ttgagatgcc aggcttcaac atcaccgtca ttgagttctt caaactgatc gtgatcgaca
      661 tcctcggagg aagatctgta agaatcgccc cagacacagc aaacaaagga atgatctctg
      721 gcctctgtgg agatcttaaa atgatggaag atacagactt cacttcagat ccagaacaac
      781 tcgctattca gcctaagatc aaccaggagt ttgacggttg tccactctat ggaaatcctg
      841 atgacgttgc atactgcaaa ggtcttctgg agccgtacaa ggacagctgc cgcaacccca
      901 tcaacttcta ctactacacc atctcctgcg ccttcgcccg ctgtatgggt ggagacgagc
      961 gagcctcaca cgtgctgctt gactacaggg agacgtgcgc tgctcccgaa actagaggaa
     1021 cctgcgtttt gtctggacat actttctacg atacatttga caaagcaaga taccaattcc
     1081 agggtccctg caaggagatt cttatggccg ccgactgttt ctggaacact tgggatgtga
     1141 aggtttcaca caggaatgtt gactcttaca ctgaagtaga gaaagtacga atcaggaaac
     1201 aatcgactgt agtagaactc attgttgatg gaaaacagat tctggttgga ggagaagccg
     1261 tgtccgtccc gtacagctct cagaacactt ccatctactg gcaagatggt gacatactga
     1321 ctacagccat cctacctgaa gctctggtgg tcaagttcaa cttcaagcaa ctgctcgtcg
     1381 tacatattag agatccattc gatggtaaga cttgcggtat ttgcggtaac tacaaccagg
     1441 atttcagtga tgattctttt gatgctgaag gagcctgtga tctgaccccc aacccaccgg
     1501 gatgcaccga agaacagaaa cctgaagctg aacgactctg caatagtctc ttcgccggtc
     1561 aaagtgatct tgatcagaaa tgtaacgtgt gccacaagcc tgaccgtgtc gaacgatgca
     1621 tgtacgagta ttgcctgagg ggacaacagg gtttctgtga ccacgcatgg gagttcaaga
     1681 aagaatgcta cataaagcat ggagacaccc tagaagtacc agatgaatgc aaataggcgg
     1741 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgtctagaa
     1801 ataattctta ctgtcatgcc aagtaagatg cttttctgtg ctgcaatagc aggcatgctg
     1861 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     1921 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1981 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2041 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc
     2101 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     2161 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2221 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2281 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2341 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2401 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2521 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2581 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2641 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2701 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     2761 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     2821 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     2881 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     2941 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3001 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3061 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3121 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3181 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     3241 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     3301 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     3361 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     3421 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     3481 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     3541 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     3601 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     3661 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3721 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3841 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     3901 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3961 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4021 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4081 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4141 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4201 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4261 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4321 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4381 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4441 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4501 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4561 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4621 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4681 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4741 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4921 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4981 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5041 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5101 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5161 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5221 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5281 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5341 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5401 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5461 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5521 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5581 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5641 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5701 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5761 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5821 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5881 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5941 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6001 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6061 c
//
