pGL2-Promoter vector (V011545)

LOCUS       pGL2-Promoter.        5790 bp DNA     circular SYN 01-JAN-1980
DEFINITION  SV40 promoter-containing vector for measuring the activity of 
            enhancer sequences with a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-Promoter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        42..238
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             268..1917
                     /label=luciferase
                     /note="firefly luciferase"
     intron          2161..2226
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2356..2376
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2801..2935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3248..3836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4010..4867)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4868..4972)
                     /label=AmpR promoter
     rep_origin      4999..5454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5644..5765
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"