LOCUS       pGL2-Promoter.        5790 bp DNA     circular SYN 01-JAN-1980
DEFINITION  SV40 promoter-containing vector for measuring the activity of 
            enhancer sequences with a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-Promoter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        42..238
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             268..1917
                     /label=luciferase
                     /note="firefly luciferase"
     intron          2161..2226
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2356..2376
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2801..2935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3248..3836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4010..4867)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4868..4972)
                     /label=AmpR promoter
     rep_origin      4999..5454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5644..5765
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctgcatctca attagtcagc
       61 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      121 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      181 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      241 gcttggcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg
      301 gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
      361 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
      421 acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
      481 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
      541 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
      601 cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
      661 gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
      721 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
      781 ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
      841 attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg
      901 catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
      961 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     1021 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     1081 ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     1141 ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     1201 attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     1261 catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     1321 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     1381 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     1441 gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     1501 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     1561 ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc
     1621 gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     1681 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     1741 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     1801 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     1861 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa
     1921 aatgtaactg tattcagcga tgacgaaatt cttagctatt gtaatactgc gatgagtggc
     1981 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg
     2041 cctgaataag tgataataag cggatgaatg gcagaaattc gccggatctt tgtgaaggaa
     2101 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     2161 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     2221 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     2281 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     2341 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     2401 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     2461 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     2521 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     2581 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     2641 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     2701 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2761 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2881 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatgatgtct ggatccgtcg
     2941 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     3001 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     3061 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3661 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4981 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5041 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5101 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5161 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5221 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5281 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5341 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5401 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt
     5461 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5521 gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt tttacttgct
     5581 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     5641 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5761 tcttatggta ctgtaactga gctaacataa
//
