pGL4.27[luc2P/minP/Hygro] vector (V011523)

LOCUS       pGL4.27[luc2P_mi        6010 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.27[luc2P minP Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..1790
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1794..1913
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1965..2086)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2280..2637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2668..3702
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3729..3777
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4104..4692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4895..5752)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5857..5905
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5919..6010
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"