LOCUS       pGL4.27[luc2P_mi        6010 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.27[luc2P minP Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..1790
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1794..1913
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1965..2086)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2280..2637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2668..3702
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3729..3777
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4104..4692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4895..5752)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5857..5905
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5919..6010
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
      301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
      361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
      421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
      481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
      541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
      601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
      661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
      721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
      781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
      841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
      901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
      961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
     1801 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
     1861 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
     1921 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     1981 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2041 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2101 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2161 cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg
     2221 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc
     2281 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     2581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     2641 ctcgattctt ctgacactag cgccaccatg aagaagcccg aactcaccgc taccagcgtt
     2701 gaaaaatttc tcatcgagaa gttcgacagt gtgagcgacc tgatgcagtt gtcggagggc
     2761 gaagagagcc gagccttcag cttcgatgtc ggcggacgcg gctatgtact gcgggtgaat
     2821 agctgcgctg atggcttcta caaagaccgc tacgtgtacc gccacttcgc cagcgctgca
     2881 ctacccatcc ccgaagtgtt ggacatcggc gagttcagcg agagcctgac atactgcatc
     2941 agtagacgcg cccaaggcgt tactctccaa gacctccccg aaacagagct gcctgctgtg
     3001 ttacagcctg tcgccgaagc tatggatgct attgccgccg ccgacctcag tcaaaccagc
     3061 ggcttcggcc cattcgggcc ccaaggcatc ggccagtaca caacctggcg ggatttcatt
     3121 tgcgccattg ctgatcccca tgtctaccac tggcagaccg tgatggacga caccgtgtcc
     3181 gccagcgtag ctcaagccct ggacgaactg atgctgtggg ccgaagactg tcccgaggtg
     3241 cgccacctcg tccatgccga cttcggcagc aacaacgtcc tgaccgacaa cggccgcatc
     3301 accgccgtaa tcgactggtc cgaagctatg ttcggggaca gtcagtacga ggtggccaac
     3361 atcttcttct ggcggccctg gctggcttgc atggagcagc agactcgcta cttcgagcgc
     3421 cggcatcccg agctggccgg cagccctcgt ctgcgagcct acatgctgcg catcggcctg
     3481 gatcagctct accagagcct cgtggacggc aacttcgacg atgctgcctg ggctcaaggc
     3541 cgctgcgatg ccatcgtccg cagcggggcc ggcaccgtcg gtcgcacaca aatcgctcgc
     3601 cggagcgcag ccgtatggac cgacggctgc gtcgaggtgc tggccgacag cggcaaccgc
     3661 cggcccagta cacgaccgcg cgctaaggag gtaggtcgag tttaaactct agaaccggtc
     3721 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc
     3781 gaactagatg ctgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     3841 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     3901 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4861 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     4921 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     4981 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     5041 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     5101 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     5161 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     5221 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     5281 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     5341 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     5401 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     5461 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     5521 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     5581 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     5641 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     5701 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc
     5761 tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa
     5821 gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta
     5881 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     5941 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     6001 acatttctct
//
