pGL4.48[luc2P/SBE/Hygro] vector (V011503)

LOCUS       pGL4.48[luc2P_SB        6052 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a Smad-binding element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.48[luc2P SBE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..332
                     /label=Smad protein complexes binding site
                     /bound_moiety="Smad protein complexes"
                     /note="SBE"
                     /note="Smad-binding element (3 copies)"
     promoter        377..408
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             441..2090
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2094..2213
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2265..2386)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2580..2937
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2968..4002
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4029..4077
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4404..4992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5195..6052)
                     /label=AmpR
                     /note="beta-lactamase"