LOCUS       pGL4.48[luc2P_SB        6052 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and a Smad-binding element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.48[luc2P SBE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..332
                     /label=Smad protein complexes binding site
                     /bound_moiety="Smad protein complexes"
                     /note="SBE"
                     /note="Smad-binding element (3 copies)"
     promoter        377..408
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             441..2090
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2094..2213
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2265..2386)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2580..2937
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2968..4002
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4029..4077
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4404..4992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5195..6052)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
       61 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
      121 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
      181 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
      241 gtgccagaac atttctctgg cctaactggc cggtacctga gctcagtatg tctagactga
      301 agtatgtcta gactgaagta tgtctagact gactcgagga tatcaagatc tggcctcggc
      361 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      421 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      481 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      541 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
      601 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
      661 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
      721 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
      781 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
      841 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
      901 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
      961 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
     1021 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
     1081 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
     1141 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
     1201 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
     1261 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1321 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     1381 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     1441 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     1501 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1561 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     1621 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     1681 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     1741 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     1801 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     1861 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     1921 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     1981 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     2041 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
     2101 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
     2161 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
     2221 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     2281 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2341 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2401 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2461 cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg
     2521 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc
     2581 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2761 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2821 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     2881 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     2941 ctcgattctt ctgacactag cgccaccatg aagaagcccg aactcaccgc taccagcgtt
     3001 gaaaaatttc tcatcgagaa gttcgacagt gtgagcgacc tgatgcagtt gtcggagggc
     3061 gaagagagcc gagccttcag cttcgatgtc ggcggacgcg gctatgtact gcgggtgaat
     3121 agctgcgctg atggcttcta caaagaccgc tacgtgtacc gccacttcgc cagcgctgca
     3181 ctacccatcc ccgaagtgtt ggacatcggc gagttcagcg agagcctgac atactgcatc
     3241 agtagacgcg cccaaggcgt tactctccaa gacctccccg aaacagagct gcctgctgtg
     3301 ttacagcctg tcgccgaagc tatggatgct attgccgccg ccgacctcag tcaaaccagc
     3361 ggcttcggcc cattcgggcc ccaaggcatc ggccagtaca caacctggcg ggatttcatt
     3421 tgcgccattg ctgatcccca tgtctaccac tggcagaccg tgatggacga caccgtgtcc
     3481 gccagcgtag ctcaagccct ggacgaactg atgctgtggg ccgaagactg tcccgaggtg
     3541 cgccacctcg tccatgccga cttcggcagc aacaacgtcc tgaccgacaa cggccgcatc
     3601 accgccgtaa tcgactggtc cgaagctatg ttcggggaca gtcagtacga ggtggccaac
     3661 atcttcttct ggcggccctg gctggcttgc atggagcagc agactcgcta cttcgagcgc
     3721 cggcatcccg agctggccgg cagccctcgt ctgcgagcct acatgctgcg catcggcctg
     3781 gatcagctct accagagcct cgtggacggc aacttcgacg atgctgcctg ggctcaaggc
     3841 cgctgcgatg ccatcgtccg cagcggggcc ggcaccgtcg gtcgcacaca aatcgctcgc
     3901 cggagcgcag ccgtatggac cgacggctgc gtcgaggtgc tggccgacag cggcaaccgc
     3961 cggcccagta cacgaccgcg cgctaaggag gtaggtcgag tttaaactct agaaccggtc
     4021 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc
     4081 gaactagatg ctgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     4141 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     4201 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4801 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5041 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5101 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5161 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     5221 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     5281 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     5341 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     5401 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     5461 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     5521 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     5581 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     5641 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     5701 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     5761 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     5821 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     5881 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     5941 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     6001 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc at
//
