pGL4.50[luc2/CMV/Hygro] vector (V011501)

LOCUS       pGL4.50[luc2_CMV        6600 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for creating stable cell lines expressing firefly luciferase 
            under control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.50[luc2 CMV Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             859..2508
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2555..2676)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2870..3227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3258..4292
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4319..4367
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4694..5282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5485..6342)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    6447..6495
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6509..6600
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"