LOCUS       pGL4.50[luc2_CMV        6600 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for creating stable cell lines expressing firefly luciferase 
            under control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.50[luc2 CMV Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             859..2508
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2555..2676)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2870..3227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3258..4292
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4319..4367
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4694..5282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5485..6342)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    6447..6495
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6509..6600
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
       61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
      781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
      841 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      901 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      961 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
     1021 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
     1081 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
     1141 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
     1201 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
     1261 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
     1321 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
     1381 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1441 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     1501 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     1561 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     1621 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     1681 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     1741 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     1801 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     1861 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     1921 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     1981 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     2041 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     2101 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     2161 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     2221 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     2281 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     2341 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     2401 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     2461 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag
     2521 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     2581 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2641 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2701 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     2761 tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg atctgcgcag
     2821 caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc tgaggcggaa
     2881 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3061 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcgattctt
     3241 ctgacactag cgccaccatg aagaagcccg aactcaccgc taccagcgtt gaaaaatttc
     3301 tcatcgagaa gttcgacagt gtgagcgacc tgatgcagtt gtcggagggc gaagagagcc
     3361 gagccttcag cttcgatgtc ggcggacgcg gctatgtact gcgggtgaat agctgcgctg
     3421 atggcttcta caaagaccgc tacgtgtacc gccacttcgc cagcgctgca ctacccatcc
     3481 ccgaagtgtt ggacatcggc gagttcagcg agagcctgac atactgcatc agtagacgcg
     3541 cccaaggcgt tactctccaa gacctccccg aaacagagct gcctgctgtg ttacagcctg
     3601 tcgccgaagc tatggatgct attgccgccg ccgacctcag tcaaaccagc ggcttcggcc
     3661 cattcgggcc ccaaggcatc ggccagtaca caacctggcg ggatttcatt tgcgccattg
     3721 ctgatcccca tgtctaccac tggcagaccg tgatggacga caccgtgtcc gccagcgtag
     3781 ctcaagccct ggacgaactg atgctgtggg ccgaagactg tcccgaggtg cgccacctcg
     3841 tccatgccga cttcggcagc aacaacgtcc tgaccgacaa cggccgcatc accgccgtaa
     3901 tcgactggtc cgaagctatg ttcggggaca gtcagtacga ggtggccaac atcttcttct
     3961 ggcggccctg gctggcttgc atggagcagc agactcgcta cttcgagcgc cggcatcccg
     4021 agctggccgg cagccctcgt ctgcgagcct acatgctgcg catcggcctg gatcagctct
     4081 accagagcct cgtggacggc aacttcgacg atgctgcctg ggctcaaggc cgctgcgatg
     4141 ccatcgtccg cagcggggcc ggcaccgtcg gtcgcacaca aatcgctcgc cggagcgcag
     4201 ccgtatggac cgacggctgc gtcgaggtgc tggccgacag cggcaaccgc cggcccagta
     4261 cacgaccgcg cgctaaggag gtaggtcgag tttaaactct agaaccggtc atggccgcaa
     4321 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc gaactagatg
     4381 ctgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     4441 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     4501 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca
     5461 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
     5521 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
     5581 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
     5641 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
     5701 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
     5761 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
     5821 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
     5881 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
     5941 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
     6001 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
     6061 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
     6121 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
     6181 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
     6241 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
     6301 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
     6361 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa
     6421 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
     6481 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     6541 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
