Basic Vector Information
- Vector Name:
- phRL-CMV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4079 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Copy Number:
- High copy number
- Promoter:
- CMV
phRL-CMV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
phRL-CMV vector Sequence
LOCUS 40924_24967 4079 bp DNA circular SYN 18-DEC-2018 DEFINITION Co-reporter vector phRL-CMV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4079) AUTHORS Zhuang Y, Kenefick KB, Kopps P. TITLE phRL-CMV Vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 4079) AUTHORS Zhuang Y, Kenefick KB, Kopps P. TITLE Direct Submission JOURNAL Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA REFERENCE 3 (bases 1 to 4079) TITLE Direct Submission REFERENCE 4 (bases 1 to 4079) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4079 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 145..523 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 524..727 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 863..995 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 1040..1058 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1068..2000 /codon_start=1 /label=hRluc /note="Renilla luciferase" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal complement(2034..2155) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 2288..2392 /label=AmpR promoter CDS 2393..3250 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3424..4012 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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