LOCUS       40924_24967        4079 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRL-CMV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     phRL-CMV Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4079)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Rd., Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        145..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          863..995
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1040..1058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1068..2000
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(2034..2155)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2288..2392
                     /label=AmpR promoter
     CDS             2393..3250
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3424..4012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcttcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat
       61 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
      121 atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat
      181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
      421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
      481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      541 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
      781 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag
      841 ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca
      901 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg
      961 tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag
     1021 ctcttaaggc tagagtactt aatacgactc actataggct agccaccatg gcttccaagg
     1081 tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca
     1141 agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga
     1201 acgccgtgat ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc
     1261 ctcacatcga gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg
     1321 gcaagagcgg gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt
     1381 tcgagctgct gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc
     1441 tggcctttca ctactcctac gagcaccaag acaagatcaa ggccatcgtc catgctgaga
     1501 gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga catcgaggag gatatcgccc
     1561 tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa taacttcttc gtcgagacca
     1621 tgctcccaag caagatcatg cggaaactgg agcctgagga gttcgctgcc tacctggagc
     1681 cattcaagga gaagggcgag gttagacggc ctaccctctc ctggcctcgc gagatccctc
     1741 tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg caactacaac gcctaccttc
     1801 gggccagcga cgatctgcct aagatgttca tcgagtccga ccctgggttc ttttccaacg
     1861 ctattgtcga gggagctaag aagttcccta acaccgagtt cgtgaaggtg aagggcctcc
     1921 acttcagcca ggaggacgct ccagatgaaa tgggtaagta catcaagagc ttcgtggagc
     1981 gcgtgctgaa gaacgagcag taattctaga gcggccgctt cgagcagaca tgataagata
     2041 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2101 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2161 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2221 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca cttttcgggg
     2281 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2341 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2401 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2461 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2521 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2581 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2641 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2701 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2761 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2821 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2881 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2941 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3001 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3061 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3121 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3181 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3241 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgac
//
