Basic Vector Information
phRL-TK(Int-) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
phRL-TK(Int-) vector Sequence
LOCUS 40924_24982 3785 bp DNA circular SYN 18-DEC-2018 DEFINITION Co-reporter vector phRL-TK(Int-), complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3785) AUTHORS Zhuang Y, Kenefick KB, Kopps P. TITLE phRL-TK(Int-) Vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 3785) AUTHORS Zhuang Y, Kenefick KB, Kopps P. TITLE Direct Submission JOURNAL Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA REFERENCE 3 (bases 1 to 3785) TITLE Direct Submission REFERENCE 4 (bases 1 to 3785) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3785 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 8..759 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 774..1706 /codon_start=1 /label=hRluc /note="Renilla luciferase" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal complement(1740..1861) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 1994..2098 /label=AmpR promoter CDS 2099..2956 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3130..3718 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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