LOCUS       40924_24982        3785 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRL-TK(Int-), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     phRL-TK(Int-) Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 3785)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3785)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Rd., Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             774..1706
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(1740..1861)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1994..2098
                     /label=AmpR promoter
     CDS             2099..2956
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3130..3718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agctctagcc accatggctt
      781 ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc
      841 gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg
      901 ccgagaacgc cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg
      961 tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta
     1021 agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg
     1081 cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg
     1141 cttgtctggc ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg
     1201 ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata
     1261 tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg
     1321 agaccatgct cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc
     1381 tggagccatt caaggagaag ggcgaggtta gacggcctac cctctcctgg cctcgcgaga
     1441 tccctctcgt taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct
     1501 accttcgggc cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt
     1561 ccaacgctat tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg
     1621 gcctccactt cagccaggag gacgctccag atgaaatggg taagtacatc aagagcttcg
     1681 tggagcgcgt gctgaagaac gagcagtaat tctagagcgg ccgcttcgag cagacatgat
     1741 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     1801 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     1861 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     1921 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccag gtggcacttt
     1981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc
     3781 tcgac
//
