Basic Vector Information
- Vector Name:
- pLightSwitch_Prom
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3656 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- SwitchGear Genomics
- Copy Number:
- High copy number
pLightSwitch_Prom vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLightSwitch_Prom vector Sequence
LOCUS pLightSwitch_Pro 3656 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for measuring promoter activity with an enhanced and
destabilized Renilla luciferase (RenSP).
ACCESSION .
VERSION .
KEYWORDS pLightSwitch_Prom.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3656)
AUTHORS SwitchGear Genomics
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3656)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3656
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..72
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 112..1044
/label=hRluc
/note="Renilla luciferase"
CDS 1048..1167
/label=hPEST
/note="PEST degradation sequence from mouse ornithine
decarboxylase"
polyA_signal complement(1210..1331)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1750..2338)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2541..3398)
/label=AmpR
/note="beta-lactamase"
polyA_signal 3503..3551
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3565..3656
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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