pLightSwitch_Prom vector (V011474)

LOCUS       pLightSwitch_Pro        3656 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for measuring promoter activity with an enhanced and 
            destabilized Renilla luciferase (RenSP).
ACCESSION   .
VERSION     .
KEYWORDS    pLightSwitch_Prom
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   SwitchGear Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..72
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             112..1044
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             1048..1167
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1210..1331)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1750..2338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2541..3398)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3503..3551
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3565..3656
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"