LOCUS       pLightSwitch_Pro        3656 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for measuring promoter activity with an enhanced and 
            destabilized Renilla luciferase (RenSP).
ACCESSION   .
VERSION     .
KEYWORDS    pLightSwitch_Prom.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   SwitchGear Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..72
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             112..1044
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             1048..1167
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1210..1331)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1750..2338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2541..3398)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3503..3551
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3565..3656
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcttac gcgtgctagc ccgggctcga gatctgcgat
       61 ctaagtaagc ttaactaagt aaggcattcc ggtactgttg gtaaagccac catggcttcc
      121 aaggtgtacg acccggagca gcgcaagagg atgatcaccg gccctcagtg gtgggctcgg
      181 tgcaagcaga tgaacgtgct cgactccttc atcaactact acgacagcga gaaacatgcg
      241 gagaacgccg tgatcttcct ccacggcaac gccgcttcct cctacctgtg gcgccacgtc
      301 gtgccccaca tcgagcccgt cgcccggtgc atcatccctg atctgatcgg gatggggaag
      361 agcgggaaga gcggcaacgg cagctaccgc ctgctcgacc actacaagta cctcaccgcc
      421 tggttcgagc tgctgaacct ccccaagaag atcatctttg tgggccacga ctggggcgct
      481 tgtctcgctt ttcactactc ctacgagcac caggataaga tcaaggctat cgtgcatgct
      541 gagagcgtcg tggacgtgat cgagtcctgg gacgagtggc ccgatatcga ggaggatatt
      601 gctctgatca agtccgagga gggcgagaag atggtcctgg agaataactt cttcgtggag
      661 actatgctgc ctagcaagat catgcgcaag ctggagcccg aggagttcgc tgcttacctg
      721 gagcccttca aggagaaggg cgaggtcaga agaccaaccc tcagctggcc tcgggagatc
      781 cctctggtca agggcgggaa gccggacgtg gtgcagatcg tccggaacta caacgcctac
      841 ctgcgcgcca gcgacgacct gcctaagatg ttcatcgagt ccgaccccgg cttcttcagc
      901 aacgctatcg tggagggcgc caagaagttc cccaacaccg agttcgtgaa ggtgaagggc
      961 ctccacttct cccaagagga cgcccctgat gagatgggga agtacatcaa gagcttcgtc
     1021 gagcgcgtcc tcaagaacga gcagaattct cacggcttcc ctcccgaggt ggaggagcag
     1081 gccgccggca ccctgcccat gagctgcgcc caggagagcg gcatggatag acaccctgct
     1141 gcttgcgcca gcgccaggat caacgtctaa tctagagtcg gggcggccgg ccgcttcgag
     1201 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1261 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1321 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     1381 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt
     1441 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     1501 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     1561 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg
     2521 ctaaaccact gcagtggtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2581 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2641 ggcttaccat ctggccccag cgctgcgatg ataccgcgag aaccacgctc accggctccg
     2701 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2761 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2821 gttaatagtt tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg
     2881 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2941 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3001 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3061 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3121 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3181 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3241 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3301 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3361 aagggaataa gggcgacacg gaaatgttga atactcatac tcgtcctttt tcaatattat
     3421 tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa tattaaggta
     3481 cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     3541 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
     3601 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct
//
