Basic Vector Information
- Vector Name:
- pMCS-Cypridina Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5989 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Thermo Scientific (Pierce)
- Copy Number:
- High copy number
- Promoter:
- SV40
pMCS-Cypridina Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMCS-Cypridina Luc vector Sequence
LOCUS pMCS-Cypridina_L 5989 bp DNA circular SYN 01-JAN-1980 DEFINITION Secreted Cypridina luciferase vector for cloning a transcriptional regulatory element. ACCESSION . VERSION . KEYWORDS pMCS-Cypridina Luc. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5989) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 5989) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5989 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..42 /label=MCS /note="MCS" /note="multiple cloning site" CDS 49..1707 /label=CLuc /note="secreted Cypridina luciferase" polyA_signal 1733..1844 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 1967..2296 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 2344..2391 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 2410..3006 /label=PuroR /note="puromycin N-acetyltransferase" polyA_signal 3139..3272 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(3316..4173) /label=AmpR /note="beta-lactamase" rep_origin 4437..5025 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5295..5311 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 5767..5783 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 5898..5989 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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