LOCUS       pMCS-Cypridina_L        5989 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Secreted Cypridina luciferase vector for cloning a transcriptional 
            regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Cypridina Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..1707
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    1733..1844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1967..2296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2344..2391
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2410..3006
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3139..3272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3316..4173)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4437..5025
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5295..5311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5767..5783
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5898..5989
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtgaat tcggtaccga gctcctcgag aagcttggat ccgccaccat gaagaccctg
       61 atcctggccg tggccctggt gtactgcgcc accgtgcact gccaggactg cccctacgag
      121 cccgaccccc ccaataccgt gcctaccagc tgcgaggcca aagagggcga atgcatcgac
      181 agcagctgcg gcacctgtac ccgggacatc ctgagcgacg gcctgtgcga gaacaagccc
      241 ggcaagacct gctgccggat gtgccagtac gtgatcgagt gccgggtgga agccgccgga
      301 tggttccgga ccttctacgg caagcggttc cagtttcagg aacccggcac ctacgtcctg
      361 ggccagggca caaagggcgg cgactggaag gtgtccatca ccctggaaaa cctggacggc
      421 accaagggcg ccgtgctgac caagaccaga ctggaagtgg ccggcgacat catcgatatc
      481 gcccaggcca ccgagaaccc catcaccgtg aacggcggag ccgaccccat cattgccaac
      541 ccctacacca tcggcgaagt gacaatcgct gtggtggaaa tgcccggctt caatatcacc
      601 gtcatcgagt tcttcaagct gatcgtgatc gacatcctgg gcggcagaag cgtgcggatc
      661 gcccccgata ccgccaacaa gggcatgatc agcggcctgt gtggcgacct gaagatgatg
      721 gaagataccg acttcaccag cgaccccgag cagctggcca tccagcccaa gatcaaccag
      781 gaatttgacg gctgccccct gtacggcaac cccgacgacg tggcctactg caagggcctg
      841 ctggaaccct acaaggacag ctgcagaaac cccatcaact tctactacta caccatcagc
      901 tgcgccttcg cccggtgcat gggcggagat gagcgcgcta gccacgtgct gctggactac
      961 agagagacat gcgccgctcc cgagacacgg ggcacatgtg tgctgagcgg ccacaccttc
     1021 tacgacacct tcgacaaggc cagataccag ttccagggcc cctgcaaaga aatcctgatg
     1081 gccgccgact gcttctggaa cacctgggac gtgaaggtgt cccaccggaa cgtggacagc
     1141 tataccgagg tggaaaaagt gcggatcaga aagcagagca ccgtggtcga gctgattgtg
     1201 gacggcaagc agatcctcgt gggcggcgag gccgtgtccg tgccctacag cagccagaac
     1261 accagcatct actggcagga cggcgacatc ctgaccaccg ccatcctgcc tgaggccctg
     1321 gtggtcaagt tcaacttcaa gcagctgctg gtggtgcaca tccgggaccc cttcgacggc
     1381 aagacatgcg gcatctgcgg caactacaac caggacttca gcgacgacag cttcgacgcc
     1441 gagggcgcct gcgacctgac ccctaatcct cccggctgca ccgaggaaca gaagcccgag
     1501 gccgagcggc tgtgcaatag cctgttcgcc ggccagagcg acctggacca gaaatgcaac
     1561 gtgtgccaca agcccgaccg ggtggaacgg tgtatgtacg agtactgcct gcggggccag
     1621 cagggcttct gcgatcacgc ctgggagttc aagaaagagt gctacatcaa gcacggcgac
     1681 accctggaag tgcccgacga gtgcaagtga gcggccgcaa aatcagcctc gactgtgcct
     1741 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1801 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcacaaca ctcaacccta
     1861 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1921 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     1981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2041 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2101 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2161 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2221 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2281 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     2341 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     2401 aactaaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     2461 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     2521 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     2581 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     2641 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     2701 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     2761 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     2821 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     2881 gccttcctgg agacatccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     2941 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     3001 ggtgcctgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     3061 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     3121 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3241 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     3301 taatcatggt cattaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3361 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3421 accatctggc cccagcgctg cgatgatacc gcgagaacca cgctcaccgg ctccggattt
     3481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3601 tagtttgcgc aacgttgttg ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4141 aataagggcg acacggaaat gttgaatact catattcttc ctttttcaat attattgaag
     4201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4261 acaaataggg gtcagtgtta caaccaatta accaattctg aacattatcg cgagcccatt
     4321 tatacctgaa tatggctcat aacacccctt gctcatgacc aaaatccctt aacgtgagtt
     4381 acgcgcgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4441 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4501 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4561 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag
     4621 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4681 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4741 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4801 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4861 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4921 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4981 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5041 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5101 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5161 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga gagtagggaa
     5221 ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa
     5281 actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg
     5341 ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat
     5401 ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt
     5461 gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat
     5521 acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt
     5581 ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat
     5641 aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata
     5701 taatacaaac tataagatgt tatcagtatt tattatcatt tagaataaat tttgtgtcgc
     5761 ccttaattgt gagcggataa caattacgag cttcatgcac agtggcgttg acattgatta
     5821 ttgactagca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5881 atgcattagt tattaataac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     5941 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctct
//
