Basic Vector Information
- Vector Name:
- pMCS-Gaussia Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4886 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Thermo Scientific (Pierce)
- Copy Number:
- High copy number
- Promoter:
- SV40
pMCS-Gaussia Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMCS-Gaussia Luc vector Sequence
LOCUS pMCS-Gaussia_Luc 4886 bp DNA circular SYN 01-JAN-1980 DEFINITION Secreted Gaussia luciferase vector for cloning a transcriptional regulatory element. ACCESSION . VERSION . KEYWORDS pMCS-Gaussia Luc. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4886) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 4886) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4886 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..42 /label=MCS /note="MCS" /note="multiple cloning site" CDS 50..604 /label=hGLuc /note="secreted Gaussia luciferase" polyA_signal 630..741 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 864..1193 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 1241..1288 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 1307..1903 /label=PuroR /note="puromycin N-acetyltransferase" polyA_signal 2036..2169 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(2213..3070) /label=AmpR /note="beta-lactamase" rep_origin 3334..3922 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4192..4208 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 4664..4680 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 4795..4886 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
This page is informational only.