LOCUS       pMCS-Gaussia_Luc        4886 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Secreted Gaussia luciferase vector for cloning a transcriptional 
            regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Gaussia Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             50..604
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
     polyA_signal    630..741
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        864..1193
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1241..1288
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1307..1903
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    2036..2169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2213..3070)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3334..3922
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4192..4208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4664..4680
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    4795..4886
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtgaat tcggtaccga gctcctcgag aagcttggat ccagccacca tgggagtcaa
       61 agttctgttt gccctgatct gcatcgctgt ggccgaggcc aagcccaccg agaacaacga
      121 agacttcaac atcgtggccg tggccagcaa cttcgcgacc acggatctcg atgctgaccg
      181 cgggaagttg cccggcaaga agctgccgct ggaggtgctc aaagagatgg aagccaatgc
      241 ccggaaagct ggctgcacca ggggctgtct gatctgcctg tcccacatca agtgcacgcc
      301 caagatgaag aagttcatcc caggacgctg ccacacctac gaaggcgaca aagagtccgc
      361 acagggcggc ataggcgagg cgatcgtcga cattcctgag attcctgggt tcaaggactt
      421 ggagcccatg gagcagttca tcgcacaggt cgatctgtgt gtggactgca caactggctg
      481 cctcaaaggg cttgccaacg tgcagtgttc tgacctgctc aagaagtggc tgccgcaacg
      541 ctgtgcgacc tttgccagca agatccaggg ccaggtggac aagatcaagg gggccggtgg
      601 tgactaagcg gccgcaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt
      661 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      721 ataaaatgag gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta
      781 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      841 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      901 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      961 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1021 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     1081 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     1141 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     1201 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     1261 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa
     1321 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     1381 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     1441 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     1501 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     1561 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     1621 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     1681 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     1741 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc
     1801 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     1861 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacacg tgctacgaga
     1921 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     1981 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     2041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2161 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat taccaatgct
     2221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga
     2341 tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg
     2401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2521 tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3061 gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa
     3181 ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac
     3241 accccttgct catgaccaaa atcccttaac gtgagttacg cgcgcgtcgt tccactgagc
     3301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3481 tcttctagtg tagccgtagt tagcccacca cttcaagaac tctgtagcac cgcctacata
     3541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4081 gtcagtgagc gaggaagcgg aaggcgagag tagggaactg ccaggcatca aactaagcag
     4141 aaggcccctg acggatggcc tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga
     4201 cggccagtct taagctcggg ccccctgggc ggttctgata acgagtaatc gttaatccgc
     4261 aaataacgta aaaacccgct tcggcgggtt tttttatggg gggagtttag ggaaagagca
     4321 tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat atatgagaat tatttaacct
     4381 tataaatgag aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa
     4441 cacctataat taaactattc atctattatt tatgattttt tgtatataca atatttctag
     4501 tttgttaaag agaattaaga aaataaatct cgaaaataat aaagggaaaa tcagtttttg
     4561 atatcaaaat tatacatgtc aacgataata caaaatataa tacaaactat aagatgttat
     4621 cagtatttat tatcatttag aataaatttt gtgtcgccct taattgtgag cggataacaa
     4681 ttacgagctt catgcacagt ggcgttgaca ttgattattg actagcatgt tctttcctgc
     4741 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taataacata
     4801 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     4861 aagtgcaggt gccagaacat ttctct
//
