Basic Vector Information
- Vector Name:
- pMCS-Gaussia-Dura Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4885 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Thermo Scientific (Pierce)
- Copy Number:
- High copy number
- Promoter:
- EM7
pMCS-Gaussia-Dura Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMCS-Gaussia-Dura Luc vector Sequence
LOCUS pMCS-Gaussia-Dur 4885 bp DNA circular SYN 01-JAN-1980 DEFINITION Secreted Gaussia-Dura luciferase vector for cloning a transcriptional regulatory element. ACCESSION . VERSION . KEYWORDS pMCS-Gaussia-Dura Luc. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4885) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 4885) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4885 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..42 /label=MCS /note="MCS" /note="multiple cloning site" CDS 49..603 /label=Gaussia-Dura Luc /note="enhanced variant of secreted Gaussia luciferase" polyA_signal 629..740 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 863..1192 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 1240..1287 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 1306..1902 /label=PuroR /note="puromycin N-acetyltransferase" polyA_signal 2035..2168 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(2212..3069) /label=AmpR /note="beta-lactamase" rep_origin 3333..3921 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4191..4207 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 4663..4679 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 4794..4885 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
This page is informational only.